'Impact of gamma rays on the germination\r'

'To find issue LD50 window glass for the shed and urther investigate the limit on sprouting and seedling parameters. The experimental terminations revealed that the part of germination had diminish after re and the effect become stronger with increase of da Gamma dose. Parameters such as germination percentage, move ot germination, mean insouciant germination, peak treasure and germination repute had significantly decreased with increased ir actin separateapy doses. Similarly seedling parameters to wit , Root distance, Shoot length, Vigour magnate and Root/Shoot length proportionality expressed higher drop-off at higher doses as comp atomic number 18d to non irradiated control.The study distinctly indicated ncrease in the deleterious do of da Gamma ir radi otherwiseapy at regular intervals, with proficiency of LD50 at a dose of 1. 50 kGy. Keywords: Gamma ir beam, potato bean, Seed germination, Groundnut (Arachis hypogaea L. ) is popularly known as peanut. It is one of the founding ‘s most popular embrocate seed crops, cultivated in more than than 100 countries of six continents. It is the single largest tooth finalize word of edible oils in India and constitutes roughly more or less 50 percent of the total oilseeds production.Among the major Groundnut growing states there has been agreeable increase in area to a lower place(a) cultivation in Andhra Pradesh. The yam bean seed mainly comprised of protein, fat, carbohydrate which make it naked as a jaybird to radioactivity induced stress. Among the environmental stresses, the radiation is the most important factor, which limits production of groundnut. This would result in drastic reduction in crop yield and magnitude of reduction would depend on groundnut varieties. non only the yield of Groundnut but also the quality of products decreases under radiation stress.The seed stage is a well-to-do phase in the plants life turn for use in radiological studies to mark off re lative radio sensitivity of species and the effects of various actors on radio sensitivity. preceding experiments in this field have indicated that ionizing radiation could cause permanent genetical effects, deadly or beneficial mutations, morphological modifications and other effects in plants. Several factors may be involved in the proscription of germination and the growth of the plants from seeds following their characterization to high beam doses.A number of radiobiological parameters are commonly used in premature assessment of effectiveness of radiation. Methods based on physiological changes such as proscription of seed germination and hoot and root elongation have been reported for detecting of irradiated legumes. Therefore, in present study the rejoinder of groundnut seed (cv. Narayan) to da Gamma radiation stress on germination and seedling parameters of groundnut was investigated compared to non irradiated seed. ASIAN J. EXP. BIOL. SCI. VOL 4 (1) 2013 61 collis ion of Gama Rays on the Seed Germination and Seedling Parameters of Groundnut (Arachis Hypogaea L. …………… M. Aparna et al. MATERIALS AND METHODS The existent for the present study comprised of seed of groundnut variety Narayan. 100 seeds were interpreted in 0. 1 mm thick polythene bags of 1 5 X 22cm dimension and ealed. The bags were exposed to gamma jibe wit n doses ot O 1. 30, 1. 50, 1. 70, 1. 90, 2. 10 and 2. 30 kGy. Samples were irradiated in continuous gamma sterilization plant (GC vitamin D0, designed by Board of ray of light and Isotope Technology, Mumbai) with 444 TBq (12000Ci) and Cobalt60 source with a specific activity of 3. 01 kGy/hour at Quality Control Laboratory, Acharya N.G. Ranga Agricultural University, Rajendranagar, Hyderabad- 500 030 and were compared with the observations made on untreated control. The material for radiation syndrome was placed in an irradiation chamber located in straight drawer inside the Lead flask. Radiati on field was provided by a situate of stationary Cobalt60 source placed in a cylindrical cage. The source was in two ways encapsulated in corrosion resistant innoxious steel pencils and was tested in symmetry with international standards. Two access holes of 8 mm diameter were provided of service sleeves for gasses, thermocouple etc. Mechanism for rotating/stirring samples during irradiation is also incorporated. The quantity of absorbed dose (kGy) can be defined as the amount of energy absorbed per social unit mass of the matter at the closure of interest. The experiment was carried out as per tout ensemble Randomized Design CRD). The irradiated seed along with nonirradiated control were sown in petridishes in the laboratory. Data on germination and seedling parameters were record seven days after sowing under ambient condition. Germination percentage was work out using the formula as per ISTA [10].Speed of germination of the given sample was figure correspond to the for mula given by Maguire [16]. Similarly, other germination parameters viz. , Mean daily germination, Peak value (Edwards [9]) and Germination value (Czebator [7]) were calculated. Seedling parameters like Shoot and root length were measured using ten seedlings ollected at random from each sample on 7th day from the seeds subjected to germination test. The require and root length were measured in centimeters (cm) using a scale and root/shoot length ratio was calculated using the estimates of seedling length.\r\n'